N-Glycan Profiling of Culture Cells

Typically, N-glycosylation studies done on cultured cells can require up to millions of cells followed by lengthy preparation to release, isolate, and profile N-glycans. To overcome these limitations, a rapid array-based workflow for profiling N-glycan signatures from cells was developed utilizing the capabilities of PNGase F-PRIME-LY™ ULTRA. This method was adapted from imaging mass spectrometry (IMS) used for on-tissue N-glycan profiling. Using this approach, N-glycan profiles from a low-density array of 8 cell chambers could be reported within four hours of completing cell culture.

Image from Angel, P. et. al. J. Proteome Res. 2019