Publications

Publications About Our Enzymes

Research is always changing, and new developments emerge quickly and staying up to date with this knowledge is a must. On this page, our team here at N-Zyme Scientifics continues to collect the latest scientific articles about our enzymes and the technologies in which we operate, to keep our customers up to date. The following is a partial list of publications citing the use of our enzymes.

  • METHODOLOGY

    • Dunne J, Griner J, Romeo M, Macdonald J, Krieg C, Lim M, Yagnik G, Rothschild KJ, Drake RR, Mehta AS, Angel PM. Evaluation of antibody-based single cell type imaging techniques coupled to multiplexed imaging of N-glycans and collagen peptides by matrix-assisted laser desorption/ionization mass spectrometry imaging. Anal Bioana Chem. 2023 Oct 16 doi: 10.1007/s00216-023-04983-2. Epub ahead of print. PMID: 37843548.
    • McDowell CT, Lu X, Mehta AS, Angel PM, Drake RR. Applications and continued evolution of glycan imaging mass spectrometry. Mass Spectrom Rev. 2023 Mar;42(2):674-705. doi: 10.1002/mas.21725. Epub 2021 Aug 15. PMID: 34392557; PMCID: PMC8946722.
    • Powers, T. W. et al. Matrix assisted laser desorption ionization imaging mass spectrometry workflow for spatial profiling analysis of N-linked glycan expression in tissues. Analytical Chemistry 85, 9799-9806. (2013). PMID: 24050758. 
    • Powers, T. W. et al. MALDI imaging mass spectrometry profiling of N-glycans in formalin fixed paraffin embedded clinical tissue blocks and tissue microarrays. PLoS One 9, e106255, pp106251-106211. (2014). PMID: 25184632.

  • CANCER STUDIES OF N-GLYCANS BY PNGASE F PRIME™

    • Blaschke, C.R.K., Hill, E.G., Mehta, A.S. et al. Integrating age, BMI, and serum N-glycans detected by MALDI mass spectrometry to classify suspicious mammogram findings as benign lesions or breast cancer. Sci Rep 12, 20801 (2022). https://doi.org/10.1038/s41598-022-25401-0. PMID: 36460712, PMCID: 9718781.
    • Young LEA, Conroy LR, Clarke HA, Hawkinson TR, Bolton KE, Sanders WC, Chang JE, Webb MB, Alilain WJ, Vander Kooi CW, Drake RR, Andres DA, Badgett TC, Wagner LM, Allison DB, Sun RC, Gentry MS. In situ mass spectrometry imaging reveals heterogeneous glycogen stores in human normal and cancerous tissues. EMBO Mol Med. 2022 Nov 8;14(11):e16029. doi: 10.15252/emmm.202216029. Epub 2022 Sep 5. PMID: 36059248; PMCID: PMC9641418.
    • Ocho-Rios S, Blaschke, CRK, Wang M, Peterson KD, DelaCourt A, Grauzam SE, Lewin D, Angel P, Roberts LR, Drake R, Mehta AS. Analysis of N-linked Glycan Alterations in Tissue and Serum Reveals Promising Biomarkers for Intrahepatic Cholangiocarcinoma. Cancer Research Communications. (2023) 3 (3): 383-394.doi: 10.1158/2767-9764.CRC-22-0422. PMID 36890858
    • Boyaval F, Dalebout H, Van Zeijl R, Wang W, Fariña-Sarasqueta A, Lageveen-Kammeijer GSM, Boonstra JJ, McDonnell LA, Wuhrer M, Morreau H, Heijs B. High-Mannose N-Glycans as Malignant Progression Markers in Early-Stage Colorectal Cancer. Cancers (Basel). 2022 Mar 18;14(6):1552. doi: 10.3390/cancers14061552. PMID: 35326703; PMCID: PMC8945895.
    • Grzeski M, Taube ET, Braicu EI, Sehouli J, Blanchard V, Klein O. In Situ N-Glycosylation Signatures of Epithelial Ovarian Cancer Tissue as Defined by MALDI Mass Spectrometry Imaging. Cancers (Basel). 2022 Feb 17;14(4):1021. doi: 10.3390/cancers14041021. PMID: 35205768; PMCID: PMC8870006.
    • Ščupáková K, Adelaja OT, Balluff B, Ayyappan V, Tressler CM, Jenkinson NM, Claes BS, Bowman AP, Cimino-Mathews AM, White MJ, Argani P, Heeren RM, Glunde K. Clinical importance of high-mannose, fucosylated, and complex N-glycans in breast cancer metastasis. JCI Insight. 2021 Dec 22;6(24):e146945. doi: 10.1172/jci.insight. 146945. PMID: 34752419
    • Heijs B. et. al. MALDI-2 for the Enhanced Analysis of N-Linked Glycans by Mass Spectrometry Imaging. Anal. Chem. 2020,92, 13904-13911. PMID: 32975931.
    • Boyaval F. et. al. N glycomic signature of stage II colorectal cancer and its association with the tumor microenvironment. Mol Cell Proteomics 2020 Oct 20:mcp.RA120.002215. doi: 10.1074/mcp.RA120.002215. PMID: 33082215.
    • Biswas, B. et. al. Transgenic Rescue of Spermatogenesis in Males With Mgat1 Deleted in Germ Cells. Frontiers in Cell an Developmental Biology 8, 212 (2020). PMID: 32300591.
    • Scott, D. A. et al. Specific N-Linked glycosylation patterns in areas of necrosis in tumor tissues. International Journal of Mass Spectrometry 437, 69-76 (2019). PMID: 31031563.
    • Herrera H, Dilday T, Uber A, Scott D, Zambrano JN, Wang M, Angel PM, Mehta AS, Drake RR, Hill EG, Yeh ES. Core-Fucosylated Tetra-Antennary N-Glycan Containing A Single N-Acetyllactosamine Branch Is Associated with Poor Survival Outcome in Breast Cancer. Int J Mol Sci. 2019 May 23;20(10):2528. PMID: 31126011
    • Briggs, M. T. et al. MALDI Mass Spectrometry Imaging of Early‐and Late‐Stage Serous Ovarian Cancer Tissue Reveals Stage‐Specific N‐Glycans. Proteomics 19, e1800482 (2019). PMID: 31364262.
    • West, C. A. et al. N-Linked glycan branching and fucosylation are increased directly in hepatocellular carcinoma tissue as determined through in situ glycan imaging. Journal of Proteome Research 17, 3454-3462. (2018). PMID: 30110170.
    • Drake, R. R. et. al. MALDI mass spectrometry imaging of NLinked glycans in tissues. Advances in Experimental Medicine and Biology 1104, 59-76 (2018). PMID: 30484244.
    • Drake, R. R. et al. MALDI Mass Spectrometry Imaging of N-Linked Glycans in Cancer Tissues. Advances in Cancer Research 134, 85-116 (2017). PMID: 28110657.
    • Holst, S. et al. Linkage-specific in-situ sialic acid derivatization for N-Glycan Mass Spectrometry Imaging of FFPE tissues. Analytical Chemistry 88, 5904-5913 (2016). PMID: 27145236.
    • Everest-Dass, A. V. et al. N-Glycan MALDI imaging mass spectrometry on formalin-fixed paraffin-embedded tissue enables the delineation of ovarian cancer tissues. Molecular & Cellular Proteomics 15, 3003-3016. (2016). PMID: 27412689.
    • Drake RR, McDowell C, West C, David F, Powers TW, Nowling T, Bruner E, Mehta AS, Angel PM, Marlow LA, Tun HW, Copland JA. Defining the human kidney N-glycome in normal and cancer tissues using MALDI imaging mass spectrometry. J Mass Spectrom. 2020 Apr;55(4):e4490. doi: 10.1002/jms.4490. Epub 2020 Jan 21. PMID: 31860772.

  • FIBROSIS STUDIES USING PNGASE F PRIME™

    • Conroy LR, Clarke HA, Allison DB, Valenca SS, Sun Q, Hawkinson TR, Young LEA, Ferreira JE, Hammonds AV, Dunne JB, McDonald RJ, Absher KJ, Dong BE, Bruntz RC, Markussen KH, Juras JA, Alilain WJ, Liu J, Gentry MS, Angel PM, Waters CM, Sun RC. Spatial metabolomics reveals glycogen as an actionable target for pulmonary fibrosis. Nat Commun. 2023 May 13;14(1):2759. doi: 10.1038/s41467-023-38437-1. PMID: 37179348.
    • Scott DA, Wang M, Grauzam S, Pippin S, Black A, Angel PM, Drake RR, Castellino S, Kono Y, Rockey DC, and Mehta AS (2022) GlycoFibroTyper: A Novel Method for the Glycan Analysis of IgG and the Development of a Biomarker Signature of Liver Fibrosis. Front. Immunol. 13:797460. doi: 10.3389/fimmu.2022.797460
    • Clift CL, Su YR, Bichell D, Jensen Smith HC, Bethard JR, Norris-Caneda K, Comte-Walters S, Ball LE, Hollingsworth MA, Mehta AS, Drake RR, Angel PM. Collagen fiber regulation in human pediatric aortic valve development and disease. Sci Rep 2021 May 7; 11(1):9751. PMID: 33963260.
    • Angel PM, Drake RR, Park, Y., Clift, CL, West, C., Berkhiser, S., Hardiman, G., Mehta, AS., Bichell, DP., Ru Su, Y. Spatial N-glycomics of the human aortic valve in development and pediatric endstage congenital aortic valve stenosis. J Mol Cell Cardiol 2021 Jan 29; 154:6-20. PMID: 33516683.
    • Angel, P. M. et al. Zonal Regulation of Collagen Types and Post-Translational Modifications In Prostatic Benign and Cancer Tissues By Imaging Mass Spectrometry. The Prostate. (2020). PMID:32687633. 
    • Clift, C. L. et.al. Multiplexed Imaging Mass Spectrometry of Histological Staining, N-Glycan and Extracellular Matrix from One Tissue Section: A Tool for Fibrosis Research. Methods in Molecular Biology (2019). PMID: 34331294
    • Angel, P. M. et al. Advances in MALDI Imaging Mass Spectrometry of Proteins in Cardiac Tissue, Including the Heart Valve. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1865, 927-935. (2017). PMID: 28341601. 

  • PROTOCOLS FOR USING PNGASE F PRIME™

    • Analyzing Integral Membrane Proteins with Simple Western
    • Grgic A, Krestensen KK, Heeren RMA. Optimized protocol for MALDI MSI of N-glycans using an on-tissue digestion in fresh frozen tissue sections. Sci Rep. 2023 Feb 16;13(1):2776. doi: 10.1038/s41598-023-29560-6. PMID: 36797298.
    • Regional N-glycan and lipid analysis for tissues using MALDI-mass spectrometry imaging
    • Voruganti S. et. al. A Detailed Protocol for Generation of Therapeutic Antibodies with Galactosylated Glycovariants at Laboratory Scale Using In-Vitro Glycoengineering Technology. J Pharm Sci. 2020 Oct 8:S0022 3549(20)30590-6. PMID: 33039440. 
    • Angel, P. M., Mehta, A. S. & Drake, R. R. Array based N-Glycan Profiling of Cells in Culture. Methods in Molecular Biology 2021 2271:331-342. PMID: 33908018.
    • Black, A. P. et al. A novel mass spectrometry platform for multiplexed N-Glycoprotein biomarker discovery from patient biofluids by antibody panel-based N-Glycan imaging. Analytical Chemistry 91, 8429-8435 (2019). PMID: 31177770. 
    • Black, A. P. et. al. Antibody Panel Based N‐Glycan Imaging for N‐Glycoprotein Biomarker Discovery. Current Protocols in Protein Science 98, e99 (2019). PMID: 31721442.
    • Angel PM. et. al. A Rapid Array-Based Approach to N-Glycan Profiling of Cultured Cells. J Proteome Res. 2019 Oct 4;18(10):3630-3639. doi: 10.1021/acs.jproteome.9b00303. Epub 2019 Sep 19. PMID: 31535553. 
    • Heijs, B. et al. Multimodal mass spectrometry imaging of N-glycans and proteins from the same tissue section. Analytical Chemistry 88, 7745-7753. (2016). PMID: 27373711. 
    • Drake, R. R. et. al. In Situ Imaging of N‐ Glycans by MALDI Imaging Mass Spectrometry of Fresh or Formalin‐Fixed Paraffin‐Embedded Tissue. Current Protocols in Protein Science 94, e68. (2018). PMID: 30074304. 
    • Angel, P. M. et. al. MALDI Imaging Mass Spectrometry of N-glycans and Tryptic Peptides from the Same Formalin-Fixed, Paraffin-Embedded Tissue Section. Methods Mol Biol. 2018;1788:225-241. doi: 10.1007/7651_2017_81. PMID: 29058228. 

  • ENDO F FOR STUDIES ON N-GLYCAN FUCOSE ISOMERS

    • Angel PM, Drake RR, Park, Y., Clift, CL, West, C., Berkhiser, S., Hardiman, G., Mehta, AS., Bichell, DP., Ru Su, Y. Spatial N-glycomics of the human aortic valve in development and pediatric endstage congenital aortic valve stenosis. J Mol Cell Cardiol 2021 Jan 29; 154:6-20. PMID: 33516683.
    • West CA, Liang H, Drake RR, Mehta AS. New Enzymatic Approach to Distinguish Fucosylation Isomers of N-Linked Glycans in Tissues Using MALDI Imaging Mass Spectrometry. J Proteome Res. 2020 Aug 7;19(8):2989-2996. doi: 10.1021/acs.jproteome.0c00024. Epub 2020 Jun 8. PMID: 32441096.

  • ADDITIONAL STUDIES OF INTEREST

    • Liu H, Chen C, Liao S, Sohaii DK, Cruz CRY, Burdo TH, Cradick TJ, Mehta A, Barrero C, Florez M, Gordon J, Grauzam S, Dressman J, Amini S, Bollard CM, Kaminski R, Khalili K. Strategic self-limiting production of infectious HIV particles by CRISPR in permissive cells. Mol Ther Nucleic Acids. 2023 May 19;32:1010-1025. doi: 10.1016/j.omtn.2023.04.027. PMID: 37346975.
    • Guo RR, Lageveen-Kammeijer GSM, Wang W, Dalebout H, Zhang W, Wuhrer M, Liu L, Heijs B, Voglmeir J. Analysis of Immunogenic Galactose-α-1,3-galactose-Containing N-Glycans in Beef, Mutton, and Pork Tenderloin by Combining Matrix-Assisted Laser Desorption/Ionization-Mass Spectroscopy and Capillary Electrophoresis Hyphenated with Mass Spectrometry via Electrospray Ionization. J Agric Food Chem. 2023 Mar 8;71(9):4184-4192. doi: 10.1021/acs.jafc.2c08067. Epub 2023 Feb 21. PMID: 36809004.
    • Weigand MR, Moore AM, Hu H, Angel PM, Drake RR, Laskin J. Imaging of N-Linked Glycans in Biological Tissue Sections Using Nanospray Desorption Electrospray Ionization (nano-DESI) Mass Spectrometry. J Am Soc Mass Spectrom. 2023 Oct 1. doi: 10.1021/jasms.3c00209. Epub ahead of print. PMID: 37779241.
    • Clarke HA, Ma X, Shedlock CJ, Medina T, Hawkinson TR, Wu L, Ribas RA, Keohane S, Ravi S, Bizon J, Burke S, Abisambra JF, Merritt M, Prentice B, Vander Kooi CW, Gentry MS, Chen L, Sun RC. Spatial Metabolome Lipidome and Glycome from a Single brain Section. bioRxiv [Preprint]. 2023 Jul 25:2023.07.22.550155. doi: 10.1101/2023.07.22.550155. PMID: 37546843.
    • Fujimoto, K., Kawamura, S., Bando, S. et al. Circulating prorenin: its molecular forms and plasma concentrations. Hypertens Res 44, 674-684 (2021). https://doi.org/10.1038/s41440-020-00610-0. PMID: 33564180.
    • Kana Hasegawa et al. Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain. Sci. Transl. Med.14,eaax7706(2022).DOI:10.1126/scitranslmed.aax7706. PMID: 35171652.
    • Yin, Tingyu, Xiaoxi Zhang, Shun Iwatani, Kazuhiko Miyanaga, and Naoyuki Yamamoto. 2023. "Uptake of Levilactobacillus brevis JCM 1059 by THP-1 Cells via Interaction between SlpB and CAP-1 Promotes Cytokine Production" Microorganisms 11, no. 2: 247. https://doi.org/10.3390/microorganisms11020247. PMID: 36838212.
    • Franzka P, Krüger L, Schurig MK, Olecka M, Hoffmann S, Blanchard V, Hübner CA. Altered Glycosylation in the Aging Heart. Front Mol Biosci. 2021 May 28;8:673044. doi: 10.3389/fmolb.2021.673044. PMID: 34124155; PMCID: PMC8194361.
    • Daiki Kobayashi, Takahiro Hiono, Osamu Ichii, Shoko Nishihara, Sayaka Takase-Yoden, Kazuo Yamamoto, Hiroto Kawashima, Norikazu Isoda, Yoshihiro Sakoda,Turkeys possess diverse Siaα2-3Gal glycans that facilitate their dual susceptibility to avian influenza viruses isolated from ducks and chickens, Virus Research, Volume 315,2022,198771,ISSN 0168-1702. PMID: 35429616
    • Hawkinson TR, Clarke HA, Young LEA, Conroy LR, Markussen KH, Kerch KM, Johnson LA, Nelson PT, Wang C, Allison DB, Gentry MS, Sun RC. In situ spatial glycomic imaging of mouse and human Alzheimer's disease brains. Alzheimers Dement. 2022 Oct;18(10):1721-1735. doi: 10.1002/alz.12523. Epub 2021 Dec 15. PMID: 34908231; PMCID: PMC9198106 (available on 2023-10-01).
    • Conroy LR, Hawkinson TR, Young LEA, Gentry MS, Sun RC. Emerging roles of N-linked glycosylation in brain physiology and disorders. Trends Endocrinol Metab. 2021 Dec;32(12):980-993. doi: 10.1016/j.tem.2021.09.006. Epub 2021 Oct 29. PMID: 34756776; PMCID: PMC8589112.
    • Haslund-Gourley BS, Grauzam S, Mehta AS, Wigdahl B, Comunale MA. Acute lyme disease IgG N-linked glycans contrast the canonical inflammatory signature. Front Immunol. 2022 Aug 5;13:949118. doi: 10.3389/fimmu.2022.949118. PMID: 35990620; PMCID: PMC9389449.
    • Veličković D, Liao YC, Thibert S, Veličković M, Anderton C, Voglmeir J, Stacey G, Zhou M. Spatial Mapping of Plant N-Glycosylation Cellular Heterogeneity Inside Soybean Root Nodules Provided Insights Into Legume-Rhizobia Symbiosis. Front Plant Sci. 2022 May 16;13:869281. doi: 10.3389/fpls.2022.869281. PMID: 35651768; PMCID: PMC9150855.
    • DelaCourt AT, Liang H, Drake RR, Angel PM, Mehta AS. Novel Combined Enzymatic Approach to Analyze Nonsialylated N-Linked Glycans through MALDI Imaging Mass Spectrometry. J Proteome Res. 2022 Jun 29. doi: 10.1021/acs.jproteome.2c00193. Epub ahead of print. PMID: 35766466.
    • Haslund-Gourley BS, Grauzam S, Mehta AS, Wigdahl B, Comunale MA. Acute lyme disease IgG N-linked glycans contrast the canonical inflammatory signature. Front Immunol. 2022 Aug 5;13:949118. Doi: 10.3389/fimmu.2022.949118. PMID: 35990620.
    • Sundararaj K, Rodgers J, Angel P, Wolf B, Nowling TK. The role of neuraminidase in TLR4-MAPK signalling and the release of cytokines by lupus serum-stimulated mesangial cells. Immunology 2021 Apr;162(4):418. PMID: 33314123.
    • Biswas KB. et. al. GPNMB is expressed in Human Epidermal Keratinocytes but disappears in the Vitiligo Lesional skin. Sci Rep. 2020 Mar 18:10(1):4930. doi: 10.1038/s41598 020-61931-1. PMID: 32188902.
    • Esteban PF. et. al. Revisiting CB1 cannabinoid receptor detection and the exploration of its interacting partners. J Neurosci Methods. 2020 May 1;337:108680. doi: 10.1016/ j-jneumeth.2020.108680. Epub Mar 4. PMID: 32145227.
    • Drake RR, McDowell C, West C, David F, Powers TW, Nowling T, Bruner E, Mehta AS, Angel PM, Marlow LA, Tun HW, Copland JA. Defining the human kidney N-Glycome in normal and cancer tissues using MALDI imaging mass spectrometry. J Mass Spectrom. 2019 Dec 20. PMID: 31860772
    • Watanabe K. et. al. Human soluble phospholipase A2 receptor is an inhibitor of the integrinmediated cell migratory response to collagen-I. Am J Physiol Cell Physiol. 2018 Sep 1;315(3):C398 C408. doi: 10.1152/ajpcell.00239.2017. PMID: 29791208.
    • Kutzner TJ. et. al. How presence of a signal peptide affects human galectins-1 and -4: Clues to explain common absence of a leader sequence among adhesion/growth-regulatory galectins. Biochim Biophys Acta Gen Subj. 2020 Jan;1864(1):129449. PMID: 31678146. 
    • Oide S. et. al. Carbohydrate-binding property of a cell wall integrity and stress response component (WSC) domain of an alcohol oxidase from the rice blast pathogen Pyricularia oryzae. Enzyme Microbial Technology. 2019 Jun;125:13-20. PMID: 30885320.
    • Everest-Dass AV. et. al. Human Disease Glycomics: Technology Advances Enabling Protein Glycosylation Analysis - Part 1. Expert Rev Proteomics: 2018 Feb;15(2):165-182. PMID: 29285957. 
    • Holst S. et. al. High Throughput and High-Sensitivity Mass Spectrometry-Based N-Glycomics of Mammalian Cells. Methods Mol Biol. 2017;1503:185-196. PMID: 27743367. 
    • Sonneveld ME. et. al. Glycosylation pattern of anti-platelet IgG is stable during pregnancy and predicts clinical outcome in Alloimmune Thrombocytopenia. Br J Haematol. 2016 Jul;174(2):310-20. PMID: 27017954. 
    • Holst S. et. al. N-glycosylation Profiling of Colorectal Cancer Cell Lines Reveals Association of Fucosylation with Differentiation and Caudal Type  Homebox 1 (CDX1)/Villin mRNA Expression. Mol Cell Proteomics. 2016 Jan;15(1):124-40. PMID: 26537799.
    • Lu X, McDowell CT, Blaschke CRK, Liu L, Grimsley G, Wisniewski L, Gao C, Mehta AS, Haab BB, Angel PM, Drake RR. Bioorthogonal Chemical Labeling Probes Targeting Sialic Acid Isomers for N-Glycan MALDI Imaging Mass Spectrometry of Tissues, Cells, and Biofluids. Anal Chem. 2023 May 16;95(19):7475-7486. doi: 10.1021/acs.analchem.2c04882. Epub 2023 May 1. PMID: 37126482; PMCID: PMC10193362
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