Compatible Glycoprotein |
Native and denatured |
Source of PNGase F PRIME-LY™ ULTRA |
Recombinant, E. coli |
Total Protocol Length |
30 minutes to 24 hours |
Typical Yield |
1 Unit of enzyme will completely deglycosylate 10 µg of denatured IgG following incubation for 10 minutes at 37°C. 1 Unit = 1 IUB milliunit. |
Quantity |
Each standard vial of lyophilized format enzyme contains 100 µg of PNGase F PRIME™ [1,000,000 Units/mL (8.14 mg/mL)] lyophilized from 20mM Tris-HCl, 50mM NaCl, pH 7.5. The final concentration of the enzyme is ultimately determined by the amount of dH2O used in the reconstitution process. |
Purity |
≥95% as determined by SDS PAGE |
Contains Glycerol |
No |
Compatible with Mass Spec |
Yes |
Affinity tag for purification |
Yes. PNGase F PRIME-LY™ ULTRA contains a His-tag. |
Reaction Conditions |
In vitro and in situ |
Storage |
-20°C or 4°C (avoid multiple freeze-thaws) |
Shelf Life |
1 year |
Microbe Free |
Yes, lot tested |