PNGase F PRIME™ - Especially Optimized for Mass Spec Imaging
| Target Core | asparagine-linked (N-linked) oligosaccharides (mannose, hybrid, and complex) |
|---|---|
| Compatible Glycoprotein | Native and denatured |
| Source of PNGase F PRIME-LY™ ULTRA | Recombinant, E. coli |
| Total Protocol Length | 30 minutes to 24 hours |
| Typical Yield | 1 Unit of enzyme will completely deglycosylate 10 µg of denatured IgG following incubation for 10 minutes at 37°C. 1 Unit = 1 IUB milliunit. |
| Quantity | Each standard vial of lyophilized format enzyme contains 100 µg of PNGase F PRIME™ [1,000,000 Units/mL (8.14 mg/mL)] lyophilized from 20mM Tris-HCl, 50mM NaCl, pH 7.5. The final concentration of the enzyme is ultimately determined by the amount of dH2O used in the reconstitution process. |
| Purity | ≥95% as determined by SDS PAGE |
| Contains Glycerol | No |
| Compatible with Mass Spec | Yes |
| Affinity tag for purification | Yes. PNGase F PRIME-LY™ ULTRA contains a His-tag. |
| Reaction Conditions | In vitro and in situ |
| Storage | -20°C or 4°C (avoid multiple freeze-thaws) |
| Shelf Life | 1 year |
| Microbe Free | Yes, lot tested |