PNGase F PRIME™ - Optimized for Mass Spec Imaging
| Target Core | Asparagine-linked (N-linked) or serine/threonine linked (O-linked) oligosaccharides (complex/terminally sialyated) |
|---|---|
| Compatible Glycoprotein | Native and denatured |
| Source of Sialidase PRIME-LY™ | recombinant, expressed in E. coli. |
| Total Protocol Length | 30 minutes to 24 hours |
| Typical Yield | 1 Unit of enzyme will completely de-sialate 10 µg of denatured alpha 1 anti-trypsin following incubation for 10 minutes at 37°C. 1 Unit = 1 IUB milliunit. |
| Concentration | 50 Units/µL after resuspending in 100 µL of dH2O. |
| Purity | ≥95% as determined by SDS PAGE |
| Contains Glycerol | No |
| Compatible with Mass Spec | Yes |
| Affinity tag for purification | Yes. Sialidase PRIME-LY™ contains a His-tag. |
| Reaction Conditions | In vitro and in situ |
| Storage | -20°C or 4°C (avoid multiple freeze-thaws) |
| Shelf Life | 1 year |
| Microbe Free | Yes, lot tested |