PNGase F PRIME™ - Especially Optimized for Mass Spec Imaging
| Target Core | alpha-1,6 linked fucosylated Asparagine-linked (N-linked) oligosaccharides (complex) |
|---|---|
| Compatible Glycoprotein | Native and denatured |
| Source of Endo F3 PRIME-LY™ | recombinant, expressed in E. Coli. |
| Total Protocol Length | 30 minutes to 24 hours |
| Typical Yield | 1 Unit of enzyme will completely deglycosylate 10 µg of denatured IgG following incubation for 10 minutes at 37°C. 1 Unit = 1 IUB milliunit. |
| Concentration | 8 Units/µL after resuspending in 100µL of dH2O. |
| Purity | ≥95% as determined by SDS PAGE |
| Contains Glycerol | No |
| Compatible with Mass Spec | Yes |
| Affinity tag for purification | Yes. Endo F3 PRIME contains a His-tag. |
| Reaction Conditions | In vitro and in situ |
| Storage | -20°C or 4°C (avoid multiple freeze-thaws) |
| Shelf Life | 1 year |
| Microbe Free | Yes, lot tested |