|
|
Compatible Glycoprotein |
Native and denatured |
Source of PNGase F PRIME™ |
Recombinant, E. coli |
Total Protocol Length |
30 minutes to 24 hours |
Typical Yield |
1 Unit of enzyme will completely deglycosylate 10 µg of denatured RNase B following incubation for 10 minutes at 37°C. 1 Unit = 1 IUB milliunit. |
Concentration |
1,000,000 units/ml (2 mg/mL) |
Purity |
≥95% as determined by SDS PAGE |
Contains Glycerol |
No |
Compatible with Mass Spec |
Yes |
Affinity tag for purifcation |
Yes. PNGase F PRIME™ contains a His-tag. |
Reaction Conditions |
In vitro and in situ |
Storage |
-20°C or 4°C (avoid multiple freeze-thaws) |
Shelf Life |
1 year |
Microbe Free |
Yes, lot tested |